MassIVE MSV000085234

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Proteomics of plasma exosomes in human cystic echinococcosis

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Description

Exosomes represent an important way of cell-cell communication. Their release into the bloodstream is similar to sending bottle messages that can safely reach (distant) target tissues and cells. In disease contexts, these vesicles provide a suitable source of markers of pathogenesis or carcinogenesis, as well as of the immune response and the host-pathogens interplay. In cystic echinococcosis (CE), the availability of a marker-based blood test would represent an extraordinary advantage for disease diagnosis, staging and follow-up. The active search for CE carriers, realized by ultrasound surveys of around 25,000 individuals in the HERACLES project, finally allowed the implementation of a proteomic biomarker discovery analysis based on plasma exosomes from CE patients. The identification of parasite proteins likely within host exosomes supports this important exchange of information and suggests possible molecular markers to be validated. Further, our study yielded valuable insights into the types of response mounted by the immune system in the presence of either active or inactive CE, supporting previous evidence with data obtained in vivo from a large, well-characterized cohort of subjects [doi:10.25345/C5MQ4V] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: echinococcosis, exosomes, plasma, biomarkers

Contact

Principal Investigators:
(in alphabetical order) 		FEDERICA FRATINI, ISS, Italia
Submitting User: federica_fratini

Publications

F. Fratini *, F. Tamarozzi, G. Macchia, L. Bertuccini, M. Mariconti, C. Birago, A. Iriarte, E. Brunett, CM. Cretu, O. Akhan, M. Siles-Lucas, A. Díaz, A. Casulli.
'Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers.
PLoS Negl Trop Dis 2020 Accepted.

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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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