MassIVE MSV000088421

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Proteomic response of Turicibacter bilis MMM721 to chicken bile and its bile acids

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Description

Bile and its individual components, mainly bile acids, are important for digestion and drive bacteria community dynamics in the upper gastrointestinal tract of chickens. However, specific responses to bile acids have been characterized in only a few commensal bacteria, and it is unclear how other members of the microbiota respond to biliary stress. Here, we used label-free LC-MS/MS to assess the proteomic response of a common inhabitant of the chicken upper intestinal tract, Turicibacter spp. MMM721, to 24 hours of growth in anaerobic growth media supplemented with 0.1% whole chicken bile, 0.1% taurochenodeoxycholic acid, or 0.1% taurocholic acid. 70, 46, and 8 differentially expressed proteins were identified in Turicibacter spp. MMM721 cultured with supplements of whole chicken bile, TCDCA, and TCA, respectively, when compared to unsupplemented controls. Many of the differentially expressed proteins were involved in ribosomal processes, post-translational modifications and chaperones, and modifications to the cell surface. To our knowledge, this work represents the first description of the Turicibacter spp. MMM721 proteomic response to bile and bile acid exposure. Ultimately, the T. bilis MMM721 response to whole bile and bile acids is highly complex, with numerous proteins from a variety of functional categories being induced. [doi:10.25345/C59P2Z] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Turicibacter, bilis, bile, taurocholate, taurochenodeoxycholate, chicken

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Principal Investigators:
(in alphabetical order) 		Torey Looft, USDA-ARS National Animal Disease Center, Food Safety and Enteric Pathogens Research Unit, USA
Submitting User: joelmaki17
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