Phosphorylation of GPA1 has been shown to functionally important in vitro and in vivo. We previously identified18 LRR RLKs transphosphorylation of AtGPA1 via in vitro kinase assay using recombinant kinase domains and AtGPA1 (Bo et al., 2018, J. Biol. Chem 293(13): 4752-4766). Among them, we here choose 12 of the kinases to map the specific sites they phosphorylated on GPA1. In vitro kinase assays were preformed with 50 uM GDP in the reaction.
Kinase # Locus No.
1 AT1G51800
2 AT4G33430
3 AT1G71830
4 AT1G72300
5 AT1G73080
6 AT3G13380
7 AT4G39400
8 AT5G49660
9 AT2G19230
10 AT2G37050
11 AT5G10290
12 AT5G62710
[doi:10.25345/C5G92Q]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: G PROTEIN ; In vitro kinase
Principal Investigators: (in alphabetical order) |
Justin Walley, Iowa State University, US |
Submitting User: | jwalley |
Jia H, Song G, Werth EG, Walley JW, Hicks LM, Jones AM.
Receptor-Like Kinase Phosphorylation of Arabidopsis Heterotrimeric G-Protein G? -Subunit AtGPA1.
Proteomics. 2019 Dec;19(24):e1900265. Epub 2019 Dec 10.
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