A defined four-member pathogen community isolated from a patient with lethal sepsis was injected directly into the peritoneum (IP) of mice. To test the protective effects of fecal microbiota transplantation (FMT) in this model, immediately upon injection of PC and then once more 14 hours post injection, FMT or an autoclaved FMT (AC-FMT) was administered via enema. Cecal contents were collected approximately 20 hours post injection of PC and short chain fatty acids were extracted from the cecal contents using diethyl ether (Fisher Scientific), derivatized using N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethylchlorosilane (Sigma). Acetate, butyrate, and propionate levels were measured by GC-MS.
[doi:10.25345/C5JQ3V]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Acetate ; Butyrate ; Proprionate ; Sepsis ; Fecal microbiota transplant
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John Alverdy, University of Chicago, USA |
Submitting User: | sangman |
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Owner | Reanalyses | |
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