MassIVE MSV000089350

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GNPS Quantification and validation (MRM) of analysis - compounds isolated from Heteropterys umbellata (Malpighiaceae)

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Description

Analyses of Heteropterys umbellata (Malpighiaceae) stems and leaves extracts. Plants were collected in two different Brazilian States (Sao Paulo and Minas Gerais). Dried plant material was extracted with EtOH80%. Positive ionization mode analyses. LC-MS/MS analyses were performed in a Shimadzu HPLC using a Phenomenex C18-Luna (250 mm x 4.6 mm, 5.0 um) column and an Amazon SL (Bruker Daltonics) mass spectrometer (ion trap) equipped with an ESI source. Multiple reaction monitoring (MRM) mode for quantification of isolated compounds. The transitions 499.10->319.10, 501.10->365.10, 602.20->466.10, and 296.10->278.00 were chosen to monitor 3,5-di-O-caffeoylquinic acid (isochlorogenic acid A); 1,2,6-tris-O-[3-nitropropanoyl]-beta-glucopyranose (karakin); 1,2,3,6-tetrakis-O-[3-nitropropanoyl]-beta-glucopyranose; and sodium diclofenac (used as internal standard), respectively. [doi:10.25345/C5PK0756M] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Malpighiaceae ; Plants ; Heteropterys umbellata ; Quantification ; MRM

Contact

Principal Investigators:
(in alphabetical order) 		Vanderlan da Silva Bolzani, Sao Paulo State University (UNESP) - Araraquara, Brazil
Submitting User: helenamrusso

Publications

Mannochio-Russo H, Nunes WDG, Almeida RF, Albernaz LC, Espindola LS, Bolzani VS.
Old Meets New: Mass Spectrometry-Based Untargeted Metabolomics Reveals Unusual Larvicidal Nitropropanoyl Glycosides from the Leaves of Heteropterys umbellata.
J Nat Prod. 2023 Mar 24;86(3):621-632. Epub 2023 Feb 27.

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GNPS content goes here (MSV000089350 [task=fd89369192d3432895c2ebaf740afee4])
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