MassIVE MSV000091408

Partial Public PXD040585

DiLeu isobaric labeling coupled with limited proteolysis mass spectrometry for high-throughput profiling of protein structural changes in Alzheimers disease

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Description

High-throughput quantitative analysis of protein conformational changes has a profound impact on our understanding of the pathological mechanisms of Alzheimers disease (AD). To establish an effective workflow enabling quantitative analysis of changes in protein conformation within multiple samples simultaneously, here we report the combination of N,N-dimethyl leucine (DiLeu) isobaric tag labeling with limited-proteolysis mass spectrometry (DiLeu-LiP-MS) for high-throughput structural protein quantitation in serum samples collected from AD patients and control donors. 23 proteins were discovered to undergo structural changes, mapping to 35 unique conformotypic peptides with significant changes between the AD group and the control group. 7 out of 23 proteins, including CO3, CO9, C4BPA, APOA1, APOA4, C1R and APOA, exhibited potential correlation with AD. Moreover, we found that complement proteins (e.g., CO3, CO9 and C4BPA) related to AD exhibited elevated levels in the AD group compared to those in the control group. These results provide evidence that the established DiLeu-LiP-MS method can be used for high-throughput structural protein quantitation, which also showed great potential in achieving large-scale and in-depth quantitative analysis of protein conformational changes in other biological systems. [doi:10.25345/C5M902C8T] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: DiLeu isobaric labeling, limited proteolysis mass spectrometry, high-throughput protein quantitation, structural protein quantitation, Alzheimers disease

Contact

Principal Investigators:
(in alphabetical order) 		Lingjun Li, University of Wisconsin-Madison, United States
Submitting User: hlu244
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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