MassIVE MSV000099310

Partial Public PXD068959

BN-SDS PAGE of Complex I from Phaeodactylum tricornutum

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Description

A native complex I-enriched fraction isolated from Phaeodactylum tricornutum was separated by two-dimensional Blue-native / SDS Polyacrylamid Gelelectrophoresis (2D BN / SDS PAGE). After vizualization of proteins on the gel with Coomassie, protein spots were cut, cysteine residues were carbamidomethylated and an in-gel digestion was carried out with trypsin overnight. Peptides were extracted and used for HPLC-tandem MS using an online EvoSep (Evosep Biosystems) - timsTOF pro MS (Bruker) system. Data were analyzed with MaxQuant (Version 2.6.8.0) and further processed in Excel (Microsoft Office). The final processed dataset (which solely focusses on complex I subunits) was used to generate a reference map at www.gelmap.de. [doi:10.25345/C53R0Q669] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Phaeodactylum, Polyacrylamid Gelelectrophoresis, Complex I, NADH ubiquinone oxidoreductase ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Jennifer Senkler, Leibniz Universitaet Hannover, Germany
Submitting User: Jennifer_Senkler
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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