MassIVE MSV000081975

Imported Reanalysis Dataset Public PXD006061

Chinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 2

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Description

Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular matrix components. We verified a method for separating gp120 from these CHO proteins with anion exchange chromatography, and assessed the biochemical activity of gp120 preparations with and without co-purified CHO proteins. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: HIV ; gp120 ; fibronectin ; extracellular matrix ; CHO ; Chinese Hamster

Contact

Principal Investigators:
(in alphabetical order) 		Shiu-Lok Hu, University of Washington, N/A
Submitting User: ccms

Publications

Plotnik D, Guo W, Cleveland B, von Haller P, Eng JK, Guttman M, Lee KK, Arthos J, Hu SL.
Extracellular Matrix Proteins Mediate HIV-1 gp120 Interactions with ?4?7.
J. Virol. Epub 2017 Oct 13.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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