MassIVE MSV000085448

Complete Public PXD019299

Cardioprotection via Endothelial Extracellular Vesicles in a Human Heart-on-Chip Ischemia-Reperfusion-Injury Model

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Description

We have mapped and analyzed the protein content of extracellular vesicles isolated from HUVECs (EEVs). Several groups of cardioprotective proteins were detected, as well as proteins related to various metabolic processes and response to stress. We have tested the effect of these EEVs on human stem cell- derived cardiomyocytes (hCMS) experiencing ischemia reperfusion injury (IRI). We have demonstrated the EEV treatment reduced cell death following IRI, and preserved contractile function. The effect depended on EEVs internalization in hCMS. We have compared protein expression profiles of hCMs treated and untreated with EEVs, under normal conditions and following IRI. We show that EEVs induce changes in the protein expression profiles of normal hCMs. The changes are related to various transcriptional and metabolic processes and localized in mainly in the nucleus and mitochondrion. After induction of IRI the differences between the EEV-treated and untreated groups are enhanced with a larger amount of differentially expressed proteins, with the most significant changes in metabolic processes and binding activity. [doi:10.25345/C5FT5Q] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Human ; HUVEC ; EVs ; human cardiomyocytes (hCMs), EEVs, IRI

Contact

Principal Investigators:
(in alphabetical order) 		Kevin Kit Parker, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, USA
Submitting User: bbudnik
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