MassIVE-KB Peptide Spectral Libraries

MassIVE KB v2: MassIVE Knowledge Base spectral library v2 - full releases

Download Library Name Description Precursors Peptides Proteins
MGF | sptxt KB 2.0.15, full Human peptide spectral library constructed from 1.25 billion spectra from in vivo proteomics and synthetic peptide experiments, including spectra from multiple data types. Use to obtain the best evidence of peptide fragmentation for all peptide variants across all included datasets. 5,948,126 2,492,495 24,677
MGF | sptxt KB 2.0.15, in vivo Human peptide spectral library constructed from 1.2 billion spectra from in vivo proteomics experiments, including spectra from multiple data types. Use to obtain the best evidence of protein existence across all included datasets. 4,843,818 1,958,922 19,436
MGF | sptxt KB 2.0.15, in vivo, HCD only Human peptide spectral library constructed from 1 billion spectra from in vivo proteomics experiments, including only high resolution spectra from label-free HCD experiments. Use for searching newly-acquired HCD data against the best representative HCD spectra for peptides detected across all included datasets. 2,140,865 1,055,165 17,421
MGF | sptxt KB 2.0.15, synthetic Human peptide spectral library constructed from 350k HCD spectra from synthetic peptides generated in the ProteomeTools and Bioplex (bait proteins) experiments. Use for searching newly-acquired HCD data against the best representative HCD spectra for synthetic peptides. 1,662,275 889,204 19,970

MassIVE KB v2: MassIVE Knowledge Base spectral library v2 - subset releases

Download Library Name Description Precursors Peptides Proteins
MGF | sptxt KB 2.0.15, in vivo, Low-res only MassIVE-KB v2, low resolution MS/MS spectra only 512,186 290,698 11,071
MGF | sptxt KB 2.0.15, in vivo, TMT only MassIVE-KB v2, MS/MS spectra of TMT-labeled peptides 777,307 462,395 13,482
MGF | sptxt KB 2.0.15, in vivo, Modified only MassIVE-KB v2, MS/MS spectra of peptides from modification-enrichment experiments 200,345 111,415 7,211
MGF | sptxt KB 2.0.15, in vivo, Multiple enzymes only MassIVE-KB v2, MS/MS spectra of peptides from proteomics experiments digested with various different enzymes 1,110,005 720,167 11,376

MassIVE KB v1: MassIVE Knowledge Base spectral library v1 - full releases

Download Library Name Description Precursors Peptides Proteins
MGF | sptxt Human HCD Spectral Library Human HCD spectral library derived from over 30TB of human MS/MS proteomics data and including ProteomeTools synthetic peptides 2,154,269 1,114,503 19,611
MGF | sptxt ProteomeTools HCD Spectral Library HCD spectral library from ProteomeTools human-derived synthetic peptides (Zolg et al, 2017, http://www.proteometools.org, PMID: 28135259) 264,836 204,625 19,552

Citation

Wang, Mingxun, Jian Wang, Jeremy Carver, Benjamin S. Pullman, Seong Won Cha, and Nuno Bandeira.
Cell systems (2018).

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