MassIVE MSV000092163

Complete Public PXD042985

Xiao_etal_TWN_Bodies_BioID_Map_VS3_2023

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Description

This dataset consists of the files for 99 raw MS files and associated peak lists and result files, all acquired on TripleTOF 6600 mass spectrometers operated in Data Dependent Acquisition (DDA) mode. All sample generation was performed by Yu-Xi Xiao. Streptavidin affinity purification was performed by Yu-Xi Xiao and Reuben Samson. Mass spectrometric acquisition was performed by Reuben Samson. Data analysis was performed by Reuben Samson and Yu-Xi Xiao. This submission contains two SAINT file data outputs that have 9 overlapping control files. The files are associated with a manuscript submitted for publication by Xiao et al. that provides insight into the involvement of TSC22D, WNK and NRBP families in the regulation cell volume in response to tonicity changes. Jason Moffat is the corresponding author of the manuscript and should be contacted for questions about this dataset (jason.moffat@sickkids.ca). [doi:10.25345/C5XP6VD4Q] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Proximity-dependent biotinylation ; BioID ; Protein-protein interaction ; Sorbitol ; Tonicity Stress ; WNK ; TWN bodies

Contact

Principal Investigators:
(in alphabetical order) 		Anne-Claude Gingras, LTRI, Canada
Submitting User: gingraslab
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.