MassIVE MSV000095444

Complete Public PXD054265

HRMS1 DIA and DDA of Lyso-IP inputs from mouse gut epithelium and stem cells

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Description

These mouse gut lysates contained three sets, with Set A including 21 DDA experiments and 21 DIA experiments, Set C including 24 DDA and 24 DIA experiments, and Set G including 27 DDA RAWs and 26 DIA RAWs (one of the Set G DDA experiments was run twice). Experiment Set A was intended to compare lysosomal compositions among ad libitum feeding, 24h fasted, and 24h fasted and re-fed conditions. Experiment Set C compared lysosomal compositions between germ-free and conventional microbiome states. Experiment Set G compared ad libitum and 24h fasted conditions within stem cells. In each case, these RAWs represent the inputs to lyso-IP, not the lysosomally-enriched samples that are produced from lyso-IP. Sample preparation was performed using the S-TRAP method, with MMTS (methyl methanethiosulfonate) alkalyting free Cys side chains and trypsin digesting C-terminal to lysines and arginines. DDA experiments collected spectra for all 120 minutes of a two hour run, while DIA experiments using the high resolution MS1 (HRMS1) technique collected scans at two different FAIMS compensation voltages for 75 minutes during each 105 minute run, with scan events ending when the B solvent of the gradient reached 45%. [doi:10.25345/C5V11VX7T] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Lyso-IP ; HRMS1

Contact

Principal Investigators:
(in alphabetical order) 		John P. LaCava, University of Groningen, Netherlands
Omer Yilmaz, Massachusetts Institute of Technology, United States of America
Submitting User: dtabb73
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