MassIVE MSV000087221

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Proteomic analysis of Fusarium sp. DS 682 Fungal Mineral Weathering in a Mineral Doped Soil Micromodel

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Description

Cell lysates of Fusarium sp. DS 682, a saprotrophic fungus, were prepared from fungal biomass grown on PDA agar without M9 and micronutrients. Fungus was exposed to minerals, 100 uL of 50% (w/v) natural kaolinite solution. Three fungal plate collections for each growth condition, with (+ mineral) and without mineral (- mineral), were grown for 14 days on PDA (incubated at 28 C) and harvested after 15 days. Extracted hyphae (500 mg) was prepared using the MPLEx protocol. A nanoACQUITY ultra performance liquid chromatography (LC) with a 2DLC system was used for separation of protein digests. Eluted peptides from the C18 column were analyzed using a Q-Exactive Plus Orbitrap MS for high resolution MS and high-energy collision-induced dissociation tandem MS by electrospray ionization for subsequent quantitative proteomic analysis. Data was searched with MaxQuant. Additional post-processed results and metadata can be accessed at https://doi.org/10.25584/KSOmicsFspDS682/1766303 [doi:10.25345/C50V4N] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: fungi ; soil micromodel ; MPLEX ; LC-MS ; peptide quantitation ; mineral weathering

Contact

Principal Investigators:
(in alphabetical order) 		Christopher R. Anderton, Pacific Northwest National Laboratory, United States
Submitting User: alchemistmatt

Publications

Bhattacharjee A, Qafoku O, Richardson JA, Anderson LN, Schwarz K, Bramer LM, Lomas GX, Orton DJ, Zhu Z, Engelhard MH, Bowden ME, Nelson WC, Jumpponen A, Jansson JK, Hofmockel KS, Anderton CR.
A Mineral-Doped Micromodel Platform Demonstrates Fungal Bridging of Carbon Hot Spots and Hyphal Transport of Mineral-Derived Nutrients.
mSystems. 2022 Dec 20;7(6):e0091322. Epub 2022 Nov 17.

Arunima Bhattacharjee, Odeta Qafoku, Jocelyn A. Richardson, Lindsey N. Anderson, Kaitlyn Schwarz, Lisa M. Bramer, Gerard X. Lomas, Daniel J. Orton, Zihua Zhu, Mark H. Engelhard, Mark E. Bowden, William C. Nelson, Ari Jumpponen, Janet K. Jansson, Kirsten S. Hofmockel, Christopher R. Anderton.
Fungal Mineral Weathering Mechanisms Revealed Through Direct Molecular Visualization.
bioRxiv, 2021 Oct. doi: https://doi.org/10.1101/2021.10.01.462718.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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