MassIVE MSV000093090

Complete Public PXD046094

Janer_et_al_ESYT1_mitochondria_ER_contacts_P129

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Description

This submission contains 24 raw mass spectrometry files and associated peak lists and result files for the manuscript by Alexandre Janer et al. that describes the proximity interactors of SMP-domain containing proteins, as well as an ER marker, an outer mitochondrial membrane marker and a tether between ER and mitochondria. BioID experiments were performed from Flp-In T-REx 293 cells and MS files were acquired on TripleTOF, Orbitrap Elite and Orbitrap Velos. MassIVE submissions corresponding to SAINT 6368 have been made. Samples were generated by Kathleen Daigneault, Mari Aaltonen and Hana Antonicka, affinity purification and mass spectrometric acquisition was performed by Zhen-Yuan Lin. Analysis was performed by Hana Antonicka under the supervision of Anne-Claude Gingras. Eric A. Shoubridge (eric.shoubridge@mcgill.ca) is the corresponding authors of the manuscript; Hana Antonicka (hana.antonicka@mcgill.ca) should be contacted for questions on this dataset. [doi:10.25345/C53J39B5Q] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: BioID ; proximity-dependent biotinylation ; mitochondria-ER contacts ; tether ; SYNJ2BP ; ESYT1

Contact

Principal Investigators:
(in alphabetical order) 		Anne-Claude Gingras, LTRI, Canada
Submitting User: gingraslab

Publications

Alexandre Janer, Jordan L. Morris, Michiel Krols, Hana Antonicka, Mari J. Aaltonen, Zhen-Yuan Lin, Hanish Anand, Anne-Claude Gingras, Julien Prudent, Eric A. Shoubridge.
ESYT1 tethers the ER to mitochondria and is required for mitochondrial lipid and calcium homeostasis.
Life Sci Alliance, accepted.

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