Brachionus rotundiformis were obtained by filtration of a non-axenic culture to remove feeder algae. Rotifers were flash frozen, ground in liquid nitrogen, and lysed by resuspension in buffer containing 0.2% NP40 with dounce homogenization. Native protein lysate was fractionated on an HPLC SEC and all fractions reduced/alkylated and digested with trypsin for LC-MS/MS. Data collection was with a 75 minute top speed DDA method on an Orbitrap fusion using HCD. Data processing was with MSBlender.
[doi:10.25345/C5G44J138]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: rotifer, SEC LECA, orbitrap fusion, Brachionus rotundiformis
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Edward Marcotte, University of Texas-Austin, United States |
Submitting User: | OpheliaP |
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