MassIVE MSV000088693

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GNPS Burkholderia thailandensis-exposed Murine Airway Epithelial and Murine Macrophage Cells and Monocultures

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Description

Extraction samples of murine airways epithelial LA-4 cells and murine macrophage RAW264.7 cells cultures exposed to different amounts of B. thailandensis for varying periods of time. Extracts of the adherent cells and their respective conditioned media were analyzed with LC-MS/MS to identify compounds detected within the aforementioned samples. Included are extracts of B. thailandensis monocultures, B. thailandensis-exposed LA-4 and RAW264.7 mammalian cells, and LA-4 and RAW264.7 unexposed monocultures. [doi:10.25345/C5SS2B] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Burkholderia, mammalian cell line metabolomics, murine macrophage, murine lung epithelial cell

Contact

Principal Investigators:
(in alphabetical order) 		Neha Garg, Georgia Institute of Technology, USA
Submitting User: naiosa

Publications

Aiosa N, Sinha A, Jaiyesimi OA, da Silva RR, Branda SS, Garg N.
Metabolomics Analysis of Bacterial Pathogen Burkholderia thailandensis and Mammalian Host Cells in Co-culture.
ACS Infect Dis. 2022 Aug 12;8(8):1646-1662. Epub 2022 Jun 29.

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GNPS content goes here (MSV000088693 [task=4b3d96d30add42508df4aceb9c739b63])
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.