Cilia were isolated from Tetrahymena thermophila SB715 by pH shock. Soluble ciliary proteins were extracted in buffer containing 1% NP40 and fractionated by HPLC SEC or mixed bed IEX. All fractions were reduced/alkylated and digested with trypsin for LC-MS/MS on an Orbitrap Fusion using a DDA 75 minute top speed CID method. Data were processed using MS Blender.
[doi:10.25345/C5GB1XS7J]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Tetrahymena thermophila, LECA, IEX, SEC, orbitrap fusion, cilia
Principal Investigators: (in alphabetical order) |
Edward Marcotte, University of Texas-Austin, United States |
Submitting User: | OpheliaP |
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