MassIVE MSV000096268

Partial Public PXD057408

Human coronavirus-229E infection alters the structure of host RNA processing complexes

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Description

The purpose of this experiment was to evaluate the human host cellular response to wild-type human coronavirus strain 229E (HCoV-229E)(ncbitaxon:11137) infection. Sample data was obtained for mock and infected (MOI 3) immortalized human lung epithelial cells (A549), immortalized human lung fibroblasts cells (MRC5), and primary human airway epithelial cells from lung tissue, and processed for proteome expression analysis using Limited Proteolysis (LiP) methods for Tandem Mass Tag 16-Plex (TMT16) and global proteomic measurements. Sample data was acquired using a Q-Exactive HF-X mass spectrometer and data was processed and compiled using MaxQuant software (v1.6.17.0). [doi:10.25345/C5XP6VF83] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: host-pathogen interactions ; human coronavirus 229E (HCoV-229E) ; limited proteolysis-based mass spectrometry (LiP-MS) ; protein structural changes ; therapeutic targets ; viral infections ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		John T. Melchior, Pacific Northwest National Laboratory, USA
Submitting User: alchemistmatt

Publications

Sarkar S, Feng S, Mitchell HD, Berger MR, Zhang T, Attah IK, Hutchinson-Bunch CM, Prozapas VN, Engbrecht K, King S, Sims AC, Melchior JT.
Human Coronavirus-229E Hijacks Key Host-Cell RNA-Processing Complexes for Replication.
J Proteome Res. 2025 Oct 3;24(10):5026-5045. Epub 2025 Sep 12.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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