We have designed an implantation-on-a-chip device to model the invasion of specialized fetal extravillous trophoblasts (EVTs) from an implanting embryo into the maternal decidua. We profiled the cellular proteome of sorted EVTs or endothelial cells from devices containing different cell compositions: 1) EVTs monoculture, 2) Endothelial cells monoculture, and 3) EVTs and endothelial cells co-cultured. The proteins were extracted using the MPLEx extraction method (Nakayasu et. al. PMCID: PMC5069757), digested with 1:50 (trypsin:protein, w:w) for 3 hr at 37 C. Solid phase extraction (SPE) was performed on the samples using 1 mL/50 mg columns from Phenomenex as previously described (PMCID: PMC7192326). Peptidic concentration was assayed using a BCA assay and 5 ul at 0.1 ug/ul were injected on the LC-MS/MS system. Data was searched with MaxQuant.
[doi:10.25345/C5VS2Z]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: implantation-on-a-chip ; trophoblasts ; uterine endothelium
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Geremy Clair, Pacific Northwest National Laboratory, United States |
Submitting User: | alchemistmatt |
Park JY, Mani S, Clair G, Olson HM, Paurus VL, Ansong CK, Blundell C, Young R, Kanter J, Gordon S, Yi AY, Mainigi M, Huh DD.
A microphysiological model of human trophoblast invasion during implantation.
Nat Commun. 2022 Mar 15;13(1):1252. Epub 2022 Mar 15.
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