Nuc-MS characterizes histone modifications and variants directly from intact
endogenous mono-nucleosomes. Preserving whole nucleosome particles enables
precise interrogation of their protein content, as for H3.3-containing nucleosomes which
had 6-fold co-enrichment of variant H2A.Z over bulk chromatin. Moreover, Nuc-MS
showed co-occurrence of oncogenic H3.3K27M with euchromatic marks (e.g., H4K16ac
and >40-fold enrichment of H3K79me2). By capturing the entire epigenetic landscape,
Nuc-MS provides a new, quantitative readout of nucleosome-level biology.
[doi:10.25345/C5G11K]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: native mass spectrometry ; top-down ; epiproteomics
Principal Investigators: (in alphabetical order) |
Neil L Kelleher, Northwestern University, United States |
Submitting User: | kelleheroffice |
Number of Files: | |
Total Size: | |
Spectra: | |
Subscribers: | |
Owner | Reanalyses | |
---|---|---|
Experimental Design | ||
Conditions:
|
||
Biological Replicates:
|
||
Technical Replicates:
|
||
Identification Results | ||
Proteins (Human, Remapped):
|
||
Proteins (Reported):
|
||
Peptides:
|
||
Variant Peptides:
|
||
PSMs:
|
||
Quantification Results | ||
Differential Proteins:
|
||
Quantified Proteins:
|
||
Browse Dataset Files | |
FTP Download Link (click to copy):
|