MassIVE MSV000087657

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Pseudomonas aeruginosa PA14 biofilms produce R-bodies, extendable protein polymers with roles in host colonization and virulence

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Description

Paper submitted to Nature Communications (2021): "Pseudomonas aeruginosa PA14 biofilms produce R-bodies, extendable protein polymers with roles in host colonization and virulence." Abstract: We used a modified R-body enrichment protocol to isolate SDS-insoluble fractions from PA14 biofilms and performed protein mass spectrometry (MS). We detected products of the reb cluster by MS. RebP1 and RebP2, which were detected in a ~1:1 ratio, were among the 12 most abundant proteins in our sample. List of Files: Q Exactive HF Delta pel1 LD1_123015_012016_98min_5uL_1.raw Delta pel2 LD2_123015_012016_98min_5uL_1.raw WT LD3_123015_98min_12uL_1_160120171453.raw Synapt G2 Delta pel1 LD1_123015_011316_5uL_1.raw LD1_123015_011316_5uL_2.raw Delta pel2 LD2_123015_011316_5uL_1.raw LD2_123015_011316_5uL_2.raw WT LD3_123015_011316_5uL_3.raw LD3_123015_011316_5uL_4.raw [doi:10.25345/C5GJ71] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: R-body, RebP1, RebP2

Contact

Principal Investigators:
(in alphabetical order) 		Lars Dietrich, Columbia University, United States
Lewis M. Brown, Columbia University, United States
Submitting User: cuproteomics
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