Determining protein targets directly bound by drug molecules remains
challenging. Here we present the isothermal shift assay, iTSA, for rapid unbiased
identification of drug targets. Compared with thermal proteome profiling, the
prevailing method for target engagement, iTSA offers a simplified workflow, 4-
fold higher throughput, and a multiplexed experimental design with higher
replication. We demonstrate its application to determination of target engagement
for several kinase inhibitors in lysates and living cells.
[doi:10.25345/C55036]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: iTSA ; proteome ; thermal shift
Principal Investigators: (in alphabetical order) |
William Old, University of Colorado Boulder, United States |
Submitting User: | wold_cub |
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