U2OS cells were split into two aliquots and cultured in one of
the following media (DMEM + 10 % dialysed FCS):
i) light medium: contains Arg-0 and Lys-0
ii) heavy medium: contains Arg-10 and Lys-8
Cells were passaged at least three times in the respective medium to ensure complete
labelling of proteins. SILAC experiments were performed in a forward and a reverse
reaction: for the forward experiment, heavy-labelled cells were irradiated with 20 Gy and
harvested 45 minutes after irradiation, while light-labelled cells were left untreated. In the
reverse experiment, labels were swapped and light-labelled cells were irradiated, while
heavy labelled cells were left untreated.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: mRNA stability ; osteosarcoma ; proteomics ; ikB kinase
Principal Investigators: (in alphabetical order) |
Klaus Scheidereit, Max Delbrueck Center, Germany |
Submitting User: | dperezh |
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Owner | Reanalyses | |
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Experimental Design | ||
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Proteins (Human, Remapped):
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Quantification Results | ||
Differential Proteins:
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Quantified Proteins:
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Browse Dataset Files | |
FTP Download Link (click to copy):
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