This dataset consists of 52 raw mass spectrometry files and associated peak lists and results files, acquired on a Thermo Orbitrap Exploris 480 operated in Data-Dependent Acquisition mode. Samples were generated by Karl Schreiber (clones were generated by Eileigh Kadijk and Karl Schreiber) and affinity purification and mass spectrometric acquisition were performed by Karl Schreiber. Analysis was performed by Karl Schreiber and Ji-Young Youn. The files are associated with a manuscript by Karl Schreiber et al. that evaluates different labeling enzymes and affinity resins for proximity-dependent biotinylation experiments. Ji-Young Youn is the corresponding author for the manuscript and should be contacted for questions regarding this dataset (jiyoung.youn@sickkids.ca).
This submission is associated with three Supplementary Files (in addition to this README file):
Table I describes the composition of this dataset (sample information)
Table II lists all the peptide identification evidence (per MSFragger)
Table III lists the SAINTexpress interactions for all analyses performed
[doi:10.25345/C5CV4C30Z]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: proximity-dependent biotinylation, BioID, miniTurbo, APEX2, TDP-43, TARDBP, Streptavidin Sepharose, MagResyn MS, SAINTexpress, proximal interactions
Principal Investigators: (in alphabetical order) |
Ji-Young Youn, Hospital for Sick Children, Canada |
Submitting User: | Younlab_Toronto |
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Experimental Design | ||
Conditions:
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Identification Results | ||
Proteins (Human, Remapped):
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Proteins (Reported):
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Peptides:
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Variant Peptides:
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PSMs:
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Quantification Results | ||
Differential Proteins:
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Quantified Proteins:
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Browse Dataset Files | Browse Results |
FTP Download Link (click to copy):
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