MassIVE MSV000086614

Complete Public PXD023205

GNPS - Experimental validation of a central carbon metabolic model in Aspergillus niger to enable metabolic engineering of a fungal cell factory

Description

The influence of selected pentose catabolic pathway (PCP) deletion strains on growth of plant biomass and re-routing of sugar catabolism was addressed to gain a better understanding of the flexibility of the fungus Aspergillus niger in using plant biomass-derived monomers. The transcriptome, metabolome and proteome response of three PCP mutant strains grown on wheat bran (WB) and sugar beet pulp (SBP) were evaluated. Proteomics samples were digested with trypsin then analyzed by LC-MS/MS with fractionation. Metabolomics samples were derivitized using a modified Fiehn protocol and analyzed by GC-MS with FAME added for retention alignment. Proteomics data was searched with MS-GF+ using PNNL's DMS Processing pipeline. [doi:10.25345/C5B49D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: lignocellulosic substrates ; pentose catabolic pathway ; D-galacturonic acid catabolic pathway ; L-rhamnose catabolic pathway, ; wheat bran ; sugar beet pulp ; CAZymes

Contact

Principal Investigators:
(in alphabetical order)
Ronald de Vries, Westerdijk Fungal Biodiversity Institute, Netherlands
Submitting User: alchemistmatt

Publications

Chroumpi T, Peng M, Markillie LM, Mitchell HD, Nicora CD, Hutchinson CM, Paurus V, Tolic N, Clendinen CS, Orr G, Baker SE, Mäkelä MR, de Vries RP.
Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates.
Front Bioeng Biotechnol. Epub 2021 Mar 8.

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GNPS content goes here (MSV000086614 [task=06130f53762e4e19ac4ea4084d6a98b6])
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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