Malaria-naive male rhesus macaques (Macaca mulatta), approximately four years of age, were inoculated intravenously with salivary gland sporozoites produced and isolated at the Centers for Disease Control and Prevention from multiple Anopheles species (An. dirus, An. gambiae, and An. stephensi) and then profiled for clinical, hematological, parasitological, immunological, functional genomic, lipidomic, proteomic, and metabolomic measurements. The experiment was designed for 100 days, and pre- and post-100 day periods to prepare subjects and administer curative treatments respectively. The anti-malarial drug artemether was subcuratively administered to all subjects at the initial peak of infection, one out of the five macaques received four additional subcurative treatments for subsequent recrudescence peaks. The experimental infection in one subject was ineffective but the macaque was followed-up for the same period of 100 days. The different clinical phases of the infection were clinically determined for each subject. Blood-stage curative doses of artemether were administered to all subjects at the end of the study. Capillary blood samples were collected daily for the measurement of CBCs, reticulocytes, and parasitemias. Capillary blood samples were collected every other day to obtain plasma for metabolomic analysis. Venous blood and bone marrow samples were collected at seven time points for functional genomic, proteomic, lipidomic, and immunological analyses. Within the MaHPIC, this project is known as 'Experiment 03'. This dataset was produced by: The MaHPIC Consortium, Monica Cabrera-Mora, Jeremy D. DeBarry, Mary R. Galinski, Jay C. Humphrey, Ebru Karpuzoglu, Jessica C. Kissinger, Regina Joice Cordy, Esmeralda VS Meyer, Alberto Moreno, Vishal Nayak, Mustafa V. Nural, Daniel S. Ory, Suman B Pakala, and Xuntian Jiang. The experimental design and protocols for this study were approved by the Emory University Institutional Animal Care and Use Committee (IACUC).
For more information on the MaHPIC, please visit http://www.systemsbiology.emory.edu/. To access other publicly available results from 'E03' and other MaHPIC Experiments, including clinical results (specifics on drugs administered, diet, and veterinary interventions), and other omics, visit http://plasmodb.org/plasmo/mahpic.jsp. This page will be updated as datasets are released to the public."[doi:10.25345/C5P047] [dataset license: CC0 1.0 Universal (CC0 1.0)]
|Principal Investigators:||Mary Galinski, Emory University, USA|
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