Mammalian spermatogenesis is associated with the transient appearance of condensed
mitochondria, a singularity of germ cells with unknown function. Using proteomic analysis,
respirometry, and electron microscopy with tomography, we studied the development of
condensed mitochondria. Condensed mitochondria arose from orthodox mitochondria
during meiosis by progressive contraction of the matrix space, which was accompanied by
an initial expansion and a subsequent reduction of the surface area of the inner membrane.
Compared to orthodox mitochondria, condensed mitochondria respired more actively,
had a higher concentration of respiratory enzymes and supercomplexes, and contained
more proteins involved in protein import and expression. After the completion of meiosis,
the abundance of condensed mitochondria declined, which coincided with the onset of the
biogenesis of acrosomes. Immuno-electron microscopy and the analysis of sub-cellular
fractions suggested that condensed mitochondria or their fragments were translocated
into the lumen of the acrosome. Thus, it seems condensed mitochondria are formed from
orthodox mitochondria by extensive transformations in order to support the formation of
the acrosomal matrix. This MassIVE dataset contains Tandem Mass Tag (TMT) peptide labeling.
[doi:10.25345/C5ST7F10D]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Acrosome, Cristae, Mitochondria, Spermatogenesis, Spermiogenesis
Principal Investigators: (in alphabetical order) |
Dr. Thomas A. Neubert, New York University School of Medicine, USA |
Submitting User: | hbromage |
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