MassIVE MSV000093893

Partial Public PXD048716

Nontargeted plasma proteomics analysis uncovers candidate biomarkers for renal disease and pulmonary hypertension in patients with sickle cell disease

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Description

Fifteen microliters of plasma from sickle cell disease patients, and pooled samples, were diluted with 5% deoxycholate and 10 mM DTT, followed by heating at 80 degC for 30 min, alkylation with 25 mM iodoacteamide and digestion with modified trypsin for 4 h at 37 degC. After acidification, samples were filtered. ~35 micrograms of each sample was separated by direction injection and microflow LC (1 mm x 100 mm Waters ACQUITY Premier; 100 uL/min) using a gradient of 3-28% acetonrile, and MS/MS was acquired on a Thermo Exploris 480 using a 120K MS1 scan and 30K MS2 with a staggered/overlapping method. Data analysis used Spectronaut 17. Study pool QC data, and individual files used for statistical analysis, are described in the accompanying metadata. [doi:10.25345/C53F4KZ9C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: data-independent acquisition ; microflow

Contact

Principal Investigators:
(in alphabetical order) 		Allison E. Ashley-Koch, Duke University, USA
Submitting User: mwfoster

Publications

Garrett ME, Foster MW, Telen MJ, Ashley-Koch AE.
Nontargeted Plasma Proteomic Analysis of Renal Disease and Pulmonary Hypertension in Patients with Sickle Cell Disease.
J Proteome Res. Epub 2024 Feb 14.

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