MassIVE MSV000093893

Partial Public PXD048716

Nontargeted plasma proteomics analysis uncovers candidate biomarkers for renal disease and pulmonary hypertension in patients with sickle cell disease

Description

Fifteen microliters of plasma from sickle cell disease patients, and pooled samples, were diluted with 5% deoxycholate and 10 mM DTT, followed by heating at 80 degC for 30 min, alkylation with 25 mM iodoacteamide and digestion with modified trypsin for 4 h at 37 degC. After acidification, samples were filtered. ~35 micrograms of each sample was separated by direction injection and microflow LC (1 mm x 100 mm Waters ACQUITY Premier; 100 uL/min) using a gradient of 3-28% acetonrile, and MS/MS was acquired on a Thermo Exploris 480 using a 120K MS1 scan and 30K MS2 with a staggered/overlapping method. Data analysis used Spectronaut 17. Study pool QC data, and individual files used for statistical analysis, are described in the accompanying metadata. [doi:10.25345/C53F4KZ9C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: data-independent acquisition ; microflow

Contact

Principal Investigators:
(in alphabetical order)
Allison E. Ashley-Koch, Duke University, USA
Submitting User: mwfoster

Publications

Garrett ME, Foster MW, Telen MJ, Ashley-Koch AE.
Nontargeted Plasma Proteomic Analysis of Renal Disease and Pulmonary Hypertension in Patients with Sickle Cell Disease.
J Proteome Res. Epub 2024 Feb 14.

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