MassIVE MSV000084105

Imported Reanalysis Dataset Public PXD014224

Comparative proteomic study on liver lipid droplets and mitochondria from mouse housed in different temperatures

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Description

Laboratory mice are standardly housed around 23ºC, setting them under chronic cold stress. Metabolic changes of liver in mice housed under thermoneutral, standard and cold temperatures remain unknown. Here we isolated lipid droplets and mitochondria from their livers for comparative proteomic study to investigate the changes. According to proteomic analysis, mitochondrial TCA cycle and retinol metabolism were enhanced while oxidative phosphorylation was no obviously affected under cold condition, suggesting liver mitochondria may increase TCA cycle capacity in biosynthetic pathways as well as retinol metabolism to help liver to adapt. Based on proteomic and immunoblotting results, Perilipin 5 and major urinary proteins were increased significantly while mitochondrial pyruvate carrier was decreased dramatically under cold condition, indicating a role they may play. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: thermoneutral temperature ; chronic cold stress ; organellar proteomics ; liver adaptation ; lipid droplet ; mitochondrion

Contact

Principal Investigators:
(in alphabetical order) 		Pingsheng Liu, Institute of Biophysics, Chinese Academy of Sciences, N/A
Submitting User: ccms

Publications

Liu Q, Zhou Z, Liu P, Zhang S.
Comparative proteomic study of liver lipid droplets and mitochondria in mice housed at different temperatures.
FEBS Lett. Epub 2019 Jun 24.

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