MassIVE MSV000089549

Partial Public

GNPS - Metabolomes of Aquimarina (Flavobacteriaceae, Bacteroidetes) Species, Negative Ionization Polarity

Description

Paper title: Insights into the antimicrobial activities and metabolomes of Aquimarina (Flavobacteriaceae, Bacteroidetes) species from the rare marine biosphere Author List: Silva, SG, Paula, P, da Silva, J.P, Mil-Homens, D, Teixeira, MC, Fialho, A, Costa, R, Keller-Costa, T PubMedID: Brief description of the data submitted: RAW Files and .mzXML Files used to generate Figures 4 and 5. Sample Collection Method: Supernatants of 120 mL of Aquimarina spp. cultures grown in 1:2 diluted MB at 24 degrees celsius in an incubator (Labtron) with 120 rpm orbital shaking for two days. Sample Extraction Method: liquid extracts, solid phase extraction (HLB cartridges), elution with methanol (100%); eluates were evaporated in a gentle flux of nitrogen gas and reconstituted in 500 microlitres of methanol-water (50:50 v/v). Ionization Souce and Polarity: Heated Electro-Spray Ionization source (HESI-II; Thermo Scientific). Negative Polarity. Chromatography and Phase: Thermo Scientific Accucore RP-18 column (2.1 times 100 mm, 2.6 microm), Water-Acetonitrile gradient with 0.1% formic acid. [doi:10.25345/C5000043R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Marine bacteria ; Bacteroidetes ; Flavobacteriaceae ; Aquimarina metabolomes ; metabolic networks

Contact

Principal Investigators:
(in alphabetical order)
Tina Keller-Costa, Institute for Bioengineering and Biosciences (iBB); Instituto Superior Tecnico, Portugal
Submitting User: Tina

Publications

Silva SG, Paula P, da Silva JP, Mil-Homens D, Teixeira MC, Fialho AM, Costa R, Keller-Costa T.
Insights into the Antimicrobial Activities and Metabolomes of Aquimarina (Flavobacteriaceae, Bacteroidetes) Species from the Rare Marine Biosphere.
Mar Drugs. Epub 2022 Jun 28.

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GNPS content goes here (MSV000089549 [task=1653ce9baaa645abb3253abd0be80e77])
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Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.