The files uploaded here are converted into a standard mzxml format, and utilize processing procedures described in 10.1021/acs.analchem.9b01682
Briefly, HXMS data processing consists of
1. MSMS experiment to identify peptides and their associated retention times. These files have *MSMS* in the filename. They are searched using HPK.fasta.
2. Unlabeled peptide pool file is used to refine the exact RT range of the peptide in the experiment (MSMS experiment may be conducted at a different time).
3. Labeling experiments are then searched using ExMS2 (see manuscript linked above) to produce carried deuterium at each time point for peptides included in the pool.
Comparing uptake patterns between "FullLength" files and "Kinase" files produces the map of differences on the kinase domain induced by the citron homology domain.
There are two CSV files, these contain the peptides identified in each MSMS run. These are found in Search Engine Files.
There are also finaltables produced by the ExMS program containing all raw and unfiltered results. There is a pdf file associated that shows the final peptides used for the analyses after discarding noisy peptides upon manual inspection.
All protection factors extracted from this data along with a summary table can be found in "File_HX_TABLES.xlsx".
[doi:10.25345/C5707X03Z]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Citron Homology Domain
Principal Investigators: (in alphabetical order) |
Benjamin Walters, Genentech, USA |
Submitting User: | btwalters |
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Owner | Reanalyses | |
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Experimental Design | ||
Conditions:
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Identification Results | ||
Proteins (Human, Remapped):
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Proteins (Reported):
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Peptides:
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Variant Peptides:
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PSMs:
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Quantification Results | ||
Differential Proteins:
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Quantified Proteins:
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Browse Dataset Files | |
FTP Download Link (click to copy):
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