Proteome, acetylome/carbamylome, and phosphoproteome of RAW 264.7 cells treated for 24 h with bacterial lipopolysaccharide. Samples were digested with trypsin in a buffer containing urea or sodium deoxycholate, labeled with tandem mass tags, and fractionated by high pH reverse phase chromatography. Samples were sequentially enriched by IMAC and anti-acetyllysine immunoaffinity capturing before analyzing by LC-MS/MS.
[doi:10.25345/C58S4K02F]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: carbamylation ; acetylation ; phosphorylation ; crosstalk ; post-translation modification
Principal Investigators: (in alphabetical order) |
Ernesto S. Nakayasu, Pacific Northwest National Laboratory, United States |
Submitting User: | alchemistmatt |
You Y, Tsai CF, Patel R, Sarkar S, Clair G, Zhou M, Liu T, Metz TO, Das C, Nakayasu ES.
Analysis of a macrophage carbamylated proteome reveals a function in post-translational modification crosstalk.
Cell Commun Signal. 2023 Sep 18;21(1):241. Epub 2023 Sep 18.
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