Description
We interrogated whether there existed a broader effect of PINK1-dependent phosphorylation after mitochondrial damage by utilizing primary cultured neurons from WT and Pink1 KO mice. Because persistent depolarization leads to mitochondrial damage {}, we treated cultured neurons with the protonophore carbonyl cyanide m-chlorophenyl hydrazine (CCCP) and blotted for PINK1. Consistent with previous studies, CCCP treatment results in an upregulation of endogenous PINK1 in WT neurons. Endogenous PINK1 is undetectable in Pink1 KO neurons with CCCP treatment.Overall, our phosphoproteome analysis of biological duplicates experiments quantified 10930 and 7207 phosphorylation sites, respectively, from the early (designated as 15-45 min) and late (designated as 2-6 hr) treatment time points
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: PINK1 phosphoproteome apoptosis BAD
Contact
Principal Investigators:
(in alphabetical order)
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Lujian Liao, Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University, China
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Submitting User: |
ddsange
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
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