MassIVE MSV000092443

Partial Public

Exposure to extracellular vesicles from Anisakis simplex induce changes in protein composition of human intestinal epithelial cell line (CACO-2) - parasite-host interaction overview

Subscribe Comment Reanalyze Spectra Add Reanalysis

Description

Anisakis simplex is one of the most prevalent parasitic nematodes (Nematoda) of marine organisms and is characterized by a complex life cycle. Humans can be accidental hosts for this parasitic species. Therefore, A. simplex was acknowledged as a biohazardous organism. The finding that nematodes can release extracellular vesicles (EVs), which are able to enter host cells, was the breakthrough discovery in parasite research. Although several approaches have been employed to study the biology of nematodes and their interactions with the host, the secretion of EVs by parasitic nematodes, as signal molecules, has been poorly studied. This led us to identify differentially regulated proteins (DRPs) between the proteome of a human intestinal epithelial cell line (CACO 2) exposed to EVs of A. simplex and the proteome of CACO 2 directly exposed to L3 larvae of A. simplex. In addition, we identified proteins present in EVs of A. simplex larvae and linked them to host proteins that they might regulate. To achieve this goal the shotgun proteomics method based on isobaric mass labeling (TMT) with a combination of nano high-performance liquid chromatography (nLC) coupled to an LTQ Orbitrap Elite mass spectrometer was used. [doi:10.25345/C5KW57V0R] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: CACO 2, Anisakis simplex, extracellular vesicles, host parasite interactions

Contact

Principal Investigators:
(in alphabetical order) 		Robert Stryinski, University of Warmia and Mazury in Olsztyn, Poland
Submitting User: robertstr
Number of Files:
Total Size:
Spectra:
Subscribers:
 
Owner Reanalyses
Experimental Design
    Conditions:
    Biological Replicates:
    Technical Replicates:
 
Identification Results
    Proteins (Human, Remapped):
    Proteins (Reported):
    Peptides:
    Variant Peptides:
    PSMs:
 
Quantification Results
    Differential Proteins:
    Quantified Proteins:
 
Browse Dataset Files
 
FTP Download Link (click to copy):

Species

Instrument

Modifications

- Dataset Reanalyses

+ Dataset History

Click here to queue conversion of this dataset's submitted spectrum files to open formats (e.g. mzML). This process may take some time.

When complete, the converted files will be available in the "ccms_peak" subdirectory of the dataset's FTP space (accessible via the "FTP Download" link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.