MassIVE MSV000092292

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Physiological/hypertensive cardiac extracellular vesicles regulate fibroblast proteome in a sex dependent manner

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Description

The composition of cardiac EVs (cEVs) isolated from hypertensive mice and their capacity to reprogram cardiac fibroblasts was investigated using mass spectrometry-based proteomic profiling. Hypertensive and recovering cEVs displayed altered presence of proteins involved in peptidase activity, response to oxygen radical, and glycerolipid metabolism. Following their uptake to cardiac fibroblasts, cEV treatment from normotensive animals resulted in potent antifibrotic and prohomeostatic functions, with sex dependent differences in RNA splicing and energy metabolism proteins.Bioinformatic analysis of hypertensive-cEV remodelled fibroblasts revealed disease associated profibrotic signalling, and sex specific influences on components involved in antioxidant activity, RNA metabolism, fatty acid metabolism, and glycosylation processing. Moreover, investigation of temporal cEV signalling across hypertension and recovery revealed dynamic sex and disease dependent reprogramming of proteins related to RNA binding, interferon signalling, protein glycosylation, ECM regulation, signal transduction, and protein and energy metabolisms. We highlight the dynamic composition and signalling of cEVs in regulating fibroblast biology, reporting sex-dependent differences in cardiac physiology and hypertension. [doi:10.25345/C57D2QJ1X] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: extacellular vesicles, heart, hypertensive

Contact

Principal Investigators:
(in alphabetical order) 		David Greening, Baker Heart & Diabetes Institute, Australia
Submitting User: dwgree
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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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