MassIVE MSV000084557

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mHtt tobacco roots proteome, QEHFX

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Description

Samples were analyzed using a nanoACQUITY UPLC system (Waters) coupled to a Q-Exactive HF-X high resolution accurate mass tandem mass spectrometer (Thermo) via a nanoelectrospray ionization source. Briefly the sample was first trapped on a Symmetry C18 180 micron x 20 mm trapping column (5 microliters/min at 99.9/0.1 v/v H2O/MeCN) followed by an analytical separation using a 1.7 micron AQCUITY HSS T3 C18 75 micron x 250 mm column (Waters) with a 90 min gradient of 5 to 30% MeCN with 0.1% formic acid at a flow rate of 400 nl/min and column temperature of 55 degC. Data collection on the HF-X MS was performed in data-dependent acquisition (DDA) mode with a 120,000 resolution (at m/z 200) full MS scan from m/z 375 to 1600 with a target AGC value of 3e6 ions and 50 ms maximum injection time (IT). The Top30 peptides were selected for MS/MS using a resolution of 15,000, max AGC of 5e4 ions and minimum AGC target of 2.25e3, max IT of 45 ms, collision energy of 27, an isolation width of 1.2 m/z and 20 s dynamic exclusion. [doi:10.25345/C5NQ3S] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: tobacco root

Contact

Principal Investigators:
(in alphabetical order) 		JiaHua Xie, NCCU, USA
Submitting User: mwfoster

Publications

Hung CY, Zhu C, Kittur FS, He M, Arning E, Zhang J, Johnson AJ, Jawa GS, Thomas MD, Ding TT, Xie J.
A plant-based mutant huntingtin model-driven discovery of impaired expression of GTPCH and DHFR.
Cell Mol Life Sci. 2022 Oct 17;79(11):553. Epub 2022 Oct 17.

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