Nucleotide sugar transporters (NSTs) are ER and Golgi-resident members of the solute carrier 35 (SLC35) family which supply substrates for glycosylation by exchanging lumenal nucleotide monophosphates for cytosolic nucleotide sugars. Defective NSTs have been associated with congenital disorders of glycosylation (CDG), however, molecular basis of many types of CDG remains poorly characterized. To better understand CDG biology, we identified potential interaction partners of UDP-galactose transporter (SLC35A2), UDP-N-acetylglucosamine transporter (SLC35A3) and an orphan nucleotide sugar transporter SLC35A4. For this purpose, each of the SLC35A2-A4 proteins was used as a bait in four independent pull-down experiments and the identity of the immunoprecipitated material was discovered using MS techniques. The consensus findings for each of the NSTs tested were listed as potential interaction partners and should prove useful as a starting point for deciphering the NST interaction network.
[doi:10.25345/C5978R]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: nucleotide sugar transporters ; UDP-galactose transporter ; UDP-N-acetylglucosamine transporter ; SLC35A subfamily of proteins ; glycosylation ; endoplasmic reticulum ; Golgi apparatus
Principal Investigators: (in alphabetical order) |
Maciej Wiktor, University of Wroclaw, Poland |
Submitting User: | maciejwiktor |
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