A spectral library was built for Solanum lycopersicum. The spectral library allows reproducible quantification for thousands of peptides per SWATH-MS analysis.
Proteins from Solanum lycopersicum pericarp were digested with trypsin using in-gel digestion and the peptides were fractionated by high-pH reverse phase chromatography. HRM peptides were spiked into the peptides mixture and each fraction was injected on a Sciex TripleTOF 6600 mass spectrometer fitted with microflow set-up.
The resulting .wiff files were analysed using MaxQuant and Spectronaut.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Spectral library ; SWATH-MS ; Solanum lycopersicum
Principal Investigators: (in alphabetical order) |
Kathryn Susan Lilley, Cambridge Centre for Proteomics, Cambridge Systems Biology Centre, University of Cambridge, United Kingdom |
Submitting User: | bertrandfabre31 |
Fabre B, Korona D, Mata-Martinez CI, Parsons HT, Deery MJ, Hertog MLATM, Nicolaï BM, Russell S, Lilley KS.
Spectral Libraries for SWATH-MS Assays for Drosophila melanogaster and Solanum lycopersicum.
Proteomics. Epub 2017 Sep 18.
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