The tryptic S peptide mixtures were analysed by by the Eksigent nanoLC-Ultra 2D System (Eksigent, AB SCIEX Dublin, CA, USA) configured in trap-elute mode as previously described(57) through a 70 min gradient of eluent B (eluent A, 0.1% formic acid in water; eluent B, 0.1% formic acid in acetonitrile). Briefly, 600 ng of digested S protein was loaded on a first trap (200-500 microm ChromXP C-18-CL, 3 microm, 120 Angstrom) and then eluted on a reverse-phase nano-column (75 microm x 15 cm ChromXP C18-CL, 3 microm, 120 Angstrom) at a flowrate of 300 nL/minute. The peptide mixtures were separated according to specific gradients (from 5-10% B in 2 min, 10-60% B in 38 min, 60-95% B in 8 min and holding in 95% B for 9 min before returning at 5% B in 3 min) and analysed by LTQ-OrbitrapXL mass spectrometer (Thermo Fisher Scientific, Waltham, CA, USA) equipped with a nanospray ion source which were managed by Xcalibur software version 1.4 (Thermo Fisher Scientific, Waltham, CA, USA). Full mass spectra were acquired at 400-1600 m/z scan range in positive ion mode and with a resolution setting of 60000 FWHM (m/z 200) and a scan rate of 2 spectra/second. The full MS was followed by five low-resolution MS/MS events that were sequentially generated in a data-dependent manner on the top five ions selected from the full MS spectrum (at 35% collision energy), with an isolation window of 2 m/z from the survey scan.
[doi:10.25345/C5D795G2C]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: UV ; Spike ; SARS-CoV-2
Principal Investigators: (in alphabetical order) |
Dario Di Silvestre, Institute of Biomedical Technologies, Italy Letizia Bernardo, Institute for Biomedical Technologies, Italy Pierluigi Mauri, Institute of Biomedical Technologies, Italy |
Submitting User: | Dds100 |
Number of Files: | |
Total Size: | |
Spectra: | |
Subscribers: | |
Owner | Reanalyses | |
---|---|---|
Experimental Design | ||
Conditions:
|
||
Biological Replicates:
|
||
Technical Replicates:
|
||
Identification Results | ||
Proteins (Human, Remapped):
|
||
Proteins (Reported):
|
||
Peptides:
|
||
Variant Peptides:
|
||
PSMs:
|
||
Quantification Results | ||
Differential Proteins:
|
||
Quantified Proteins:
|
||
Browse Dataset Files | |
FTP Download Link (click to copy):
|