MassIVE MSV000092995

Complete Public PXD045773

Generation and Characterization of a Monoclonal Antibody Against an African Swine Fever Virus Protein Encoded by the A137R Gene

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Description

The ongoing African swine fever (ASF) pandemic continues to have a major impact on global pork production and trade. Since ASF cannot be distinguished from other swine hemorrhagic fevers clinically, ASF-specific laboratory diagnosis is critical. Thus ASF virus (ASFV)-specific monoclonal antibodies (mAbs) are critical for the development of laboratory diagnostics. In this study, we report one ASFV-specific mAb, F88ASF-55, that was generated and characterized. A proteomic approach was performed to determine the sequence identities of the protein band (14 kDa) recognized by F88ASF-55. The stained protein band was excised, digested, and analyzed by LC-MS/MS. A total of 10 exclusive unique peptides and 15 exclusive unique spectra were identified (data not shown). The amino acid coverage (88/137) of the structural protein encoded by A137R was 64.2%. The results confirmed that the mAb-binding epitope is located on the protein encoded by A137R (formerly known as p11.5). Epitope mapping results revealed a highly conserved linear epitope recognized by this mAb, corresponding to amino acids 111-125 of pA137R. [doi:10.25345/C51834C85] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: African swine fever virus ; monoclonal antibody ; antibody binding epitope ; double-antibody sandwich ELISA ; immunohistochemistry ; in situ hybridization

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Principal Investigators:
(in alphabetical order) 		Ming Yang, Canadian Food Inspection Agency, Canada
Submitting User: yangm
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