MassIVE MSV000094564

Partial Public PXD051561

High-density CRISPRi screens reveal adaptive transcriptional gradients in cyanobacteria

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Description

Cyanobacteria are the oldest form of photosynthetic life and contribute to primary production in nearly every habitat, from permafrost to hot springs. Despite longstanding interest in the biochemical basis of environmental adaptation in these microbes, it remains poorly understood. Here we used a high-density, genome-wide CRISPRi screen to examine the influence of gene-specific transcriptional variation on the growth of Synechococcus sp. PCC 7002 under environmental extrema. Surprisingly, many partial knockdowns enhanced fitness under cold monochromatic conditions. Notably, transcriptional repression of a gene for a core subunit of the NDH-1 complex, which is important for photosynthesis and carbon uptake, improved growth rates under both red and blue light but at distinct, color-specific optima. Multi-target transcriptional repression produced nonadditive effects. Findings reveal diverse mechanisms of environmental adaptation in cyanobacteria and provide a new approach for using gradients in sgRNA activity to pinpoint biochemically influential transcriptional changes in cells. [doi:10.25345/C5J38KV37] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Cyanobacteria ; Proteomics

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Principal Investigators:
(in alphabetical order) 		Paul Abraham, ORNL, United States
Submitting User: dlcarper
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