Description
Nanomaterials in blood must mitigate the immune response to have prolonged residency, a property that is highly relevant to biocompatibility, toxicity, and the generation of long acting therapeutics. The composition of the protein corona that forms at the nano-biointerface may be directing this, however, it is currently unknown the possible correlation of corona composition with blood residency. We developed a panel of new soft single molecule polymer nanomaterials (SMPNs) with varying circulation times in mice (t1/2 ~22 to 65 h) and used proteomics to probe the nano-biointerface to elucidate the mechanism of blood residency of nanomaterials. The composition of the protein opsonins on SMPNs was qualitatively and quantitatively dynamic with time in circulation, and SMPNs that circulated longer were able to clear some of the initial surface-bound common opsonins, including immunoglobulins, complement, and coagulation proteins. This continuous remodelling of protein opsonins, a phenomenon first of its kind observed, may be a decisive step in directing elimination or residence of the reported soft nanomaterials in vivo.
[doi:10.25345/C5NX3V]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Circulating nanoparticle
Contact
Principal Investigators:
(in alphabetical order)
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Jayachandran N. Kizhakkedathu, University of British Columbia, Canada
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Submitting User: |
Rogy
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Number of distinct biological replicates across all analyses (original submission and reanalyses)
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"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
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Number of distinct unmodified peptide sequences reported across all analyses (original
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Number of distinct peptide sequences (including modified variants or peptidoforms) reported
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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
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Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.