MassIVE MSV000085863

Complete Public PXD020679

Proteomic signatures of corals from thermodynamic reefs

Description

In this work, I took an iTRAQ approach to profile the proteomes of 12 coral samples. Specifically, corals were collected from an upwelling site in the Luzon Strait ("Houbihu") and a non-upwelling "control" site in the nearby Taiwan Strait ("Houwan") and exposed to either highly variable (upwelling-simulating) or stable temperatures in the laboratory for seven days prior to sacrificing for protein analysis (the "Seriatopora hystrix variable temperature study" [SHVTS]). In this submission, I have uploaded the manuscript describing the experiment (Mayfield et al. 2012 J Exp Biol), the proofs of the manuscript describing the proteomic data (Mayfield in press Microorganisms), two RAW files (SHVTS-A & SHVTS-B for iTRAQ batches A & B, respectively), two MZML files (SHVTS-A & SHVTS-B), two MZID files (SHVTS-A & SHVTS-B), and a tab-delineated file that describes which samples were labelled with which iTRAQ tag ("Mayfield online supplemental data file"). I have also uploaded the supplemental material that accompanies the Mayfield (in press) article since that is what readers interested in the specifics of the proteomic analysis pipeline should consult. The peak data were queried against a conceptually translated S. hystrix-Cladocopium dinoflagellate transcriptome that has been uploaded as a fasta file. Please note that all proteomic data represent a mix of multiple organisms (namely the anthozoan host and the dinoflagellate endosymbionts that inhabit their gastrodermal cells). All data analysis was conducted with Proteome Discoverer (Thermo-Fisher Scientific). [doi:10.25345/C5P46C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: coral reefs ; dinoflagellates ; proteomics ; iTRAQ ; symbiosis ; thermal biology

Contact

Principal Investigators: Anderson Mayfield, University of Miami, United States
Submitting User: abmayfield

Publications

Mayfield AB.
Proteomic Signatures of Corals from Thermodynamic Reefs.
Mayfield AB (2020) Proteomic Signatures of Corals from Thermodynamic Reefs. Microorganisms (in press).

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.