MassIVE MSV000082216

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Chemical-mediated digestion: an alternative realm for middle-down proteomics?

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Description

Generating longer peptides is a paramount step in launching a middle-down proteomics pipeline, but the quest for the selection of a cleaving agent that would provide the desired 3-15 kDa peptides remains open. Here we describe the use of chemical reagent for a middle-down pipeline. Particularly we investigated CNBr, o-iodosobenzoic acid, BNPS-Skatole and, most importantly, NTCB as potential cleaving agents targeting rare amino acid residues. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: chemical digestion ; NTCB ; CNBr ; BNPS-Skatole ; iodosobenzoic acid ; middle-down ; Orbitrap

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Principal Investigators:
(in alphabetical order) 		Yury O. Tsybin, Spectroswiss Sarl, Switzerland
Submitting User: Luca_Fornelli
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