MassIVE MSV000094491

Partial Public PXD051276

GNPS - Metabolomics reveals the importance of metabolites in Mussaenda pubescens for antioxidant properties and quality traits

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Description

Mussaenda pubescens (Mp) is a valuable medicinal plant that has traditionally been used for medicinal purposes or as a tea substitute. However, there are few studies on the comprehensive and dynamic evaluation of Mp metabolites presented in Mp. This study used an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach and biochemical analysis to investigate substance changes in leaves at three different stages and elucidate the relationship between metabolites and antioxidant capacity. The findings showed that MpP leaves contained 957 metabolites, the majority of which were phenolic acids, lipids, and terpenoids. The metabolite profiling of Mp leaves was significantly influenced by their growth and development at different stages. A total of 317 differentially accumulated metabolites (DAMs) were screened, including 150 primary metabolites and 167 secondary metabolites, with 202 DAMs found in bud leaf vs. tender leaf, 54 DAMs in tender leaf vs. mature leaf, and 254 DAMs in bud leaf vs. mature leaf. Total phenolics, flavonoids, and anthocyanin concentrations decreased as Mp leaves grew and developed, whereas terpenoids increased significantly. The secondary metabolites also demonstrated a positive correlation with antioxidant activity. Phenolics, flavonoids, terpenoids, and anthocyanins were the primary factors influencing the antioxidant activity of leaves. These findings provide new insights into the metabolite formation mechanism, as well as the development and utilization of Mp tea. [doi:10.25345/C5ZK55Z0K] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Metabolomics ; Mussaenda pubescens ; antioxidant ; Leave growth stages

Contact

Principal Investigators:
(in alphabetical order) 		Caibi Zhou, Qiannan Normal University for Nationalities, China
Teerayoot Girdthai, Suranaree University of Technology, Thailand
Xiaolu Zhou, Qiannan Normal University for Nationalities, China
Submitting User: TeerayootGirdthai
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