MassIVE MSV000085628

Complete Public PXD020003

Kawashima_Phosphorylation_PHLPP1_2020_TripleTOF6600

Description

This dataset consists of 20 raw MS files and associated peak lists and result files, acquired on AB SCIEX 6600 TripleTOF mass spectrometer operated in Data Dependent Acquisition mode. Samples were generated by Cassandra Wong. Affinity purifications, mass spectrometry acquisition and analysis were also performed by Cassandra Wong under the supervision Anne-Claude Gingras. The files are associated with a manuscript submitted for publication by Agnieszka T. Kawashima et al. The main goal of this paper was to identify cell cycle dependent regulation of PHLPP1 and its interaction with mitotic proteins. Alexandra Newton is the corresponding author of the manuscript (anewton@health.ucsd.edu); Anne-Claude Gingras should be contacted for questions on this dataset (gingras@lunenfeld.ca). [doi:10.25345/C5XQ51] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: BioID ; Proximity-dependent biotinylation ; Mitosis ; PHLPP1

Contact

Principal Investigators:
(in alphabetical order)
Anne-Claude Gingras, LTRI, Canada
Submitting User: gingraslab

Publications

Agnieszka T. Kawashima,a,b Cassandra Wong,c Gema Lordén,a Charles C. King,e Pablo Lara-Gonzalez,f Arshad Desai,f Anne-Claude Gingras,c,d and Alexandra C. Newton.
The PHLPP1 N-Terminal Extension Is a Mitotic Cdk1 Substrate and Controls an Interactome Switch.
Mol Cell Biol . 2021 Feb 23;41(3):e0033320. doi: 10.1128/MCB.00333-20. Epub 2021 Jan 4.

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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.