MassIVE MSV000091514

Partial Public PXD040985

Beta-hydroxybutyrate is a metabolic regulator of proteostasis in aging and Alzheimer disease

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Description

Beta-hydroxybutyrate (BHB) is a ketone body whose signaling effects are not well characterized. The Newman Lab has observed a shift in brain cellular protein solubility following BHB treatment in vitro and in vivo. In the brains of C57BL/6 mice that received a 7-day oral gavage of ketone ester, we have observed formation of insoluble aggregates compared to control (canola oil). In a separate analysis, we treated soluble neuronal lysate from older C57BL/6 mice with multiple concentrations of R-BHB, then separated the proteins which become insolubilized. Here we seek to understand the composition of these insoluble aggregates by mass spectrometry and which proteins remain soluble, as a function of R-BHB treatment concentration. [doi:10.25345/C5SB3X81G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: proteostasis ; insoluble aggregates ; proteomics ; DIA-MS ; Beta-hydroxybutyrate (BHB) treatment

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Principal Investigators:
(in alphabetical order) 		Birgit Schilling, Buck Institute, USA
Submitting User: cking
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