Description
SETD1A, a Set1/COMPASS family member maintaining histone-H3-lysine-4 (H3K4) methylation on transcriptionally active promoters, is overexpressed in breast cancer. Here we show that SETD1Asupports mitotic processes and consequentially, its knockdown induces senescence, independent of mutations in p53, RBand p16, known senescence mediators.
The following labeling scheme was used:
bridge_1; TMT1_126
A549_GFPctrl_Day3_Repl1; TMT1_127n
A549_shSETD1A#1_Day3_Repl1; TMT1_127c
A549_shSETD1A#2_Day3_Repl1; TMT1_128n
A549_GFPctrl_Day5_Repl1; TMT1_128c
A549_shSETD1A#1_Day5_Repl1; TMT1_129n
A549_shSETD1A#2_Day5_Repl1; TMT1_129c
M231_GFPctrl_Day3; TMT1_130n
M231_GFP_ctrl_Day5; TMT1_130c
bridge_2; TMT1_131
bridge_1; TMT2_126
A549_GFP_ctrl_Day3_Repl2; TMT2_127n
A549_shSETD1A#1_Day3_Repl2; TMT2_127c
A549_shSETD1A#2_Day3_Repl2; TMT2_128n
A549_GFPctrl_Day5_Repl2; TMT2_128c
A549_shSETD1A#1_Day5_Repl2; TMT2_129n
A549_shSETD1A#2_Day5_Repl2; TMT2_129c
M231_shSETD1A#2_Day3; TMT2_130n
M231_shSETD1A#2_Day5; TMT2_130c
bridge_2; TMT2_131
[doi:10.25345/C58D2W]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: SETD1A, human cell lines, full proteome, TMT10, SPS-MS3, Orbitrap Fusion
Contact
Principal Investigators:
(in alphabetical order)
|
Shyamala Maheswaran, Massachusetts General Hospital Cancer Center and Harvard Medical School Boston, USA
|
Submitting User: |
whaas
|
Number of Files: |
|
Total Size: |
|
Spectra: |
|
Subscribers: |
|
|
| Owner |
Conditions:
|
|
|
Biological Replicates:
|
|
|
Technical Replicates:
|
|
|
|
Identification Results |
Proteins (Human, Remapped):
|
|
|
Proteins (Reported):
|
|
|
Peptides:
|
|
|
Variant Peptides:
|
|
|
PSMs:
|
|
|
|
Differential Proteins:
|
|
|
Quantified Proteins:
|
|
|
|
Click here to queue conversion of this dataset's submitted spectrum files
to open formats (e.g. mzML). This process may take some time.
When complete, the converted files will be available in the "ccms_peak"
subdirectory of the dataset's FTP space (accessible via the "FTP Download"
link to the right).
Number of distinct conditions across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct condition labels are counted across all files submitted in the "Metadata" category
having a "Condition" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct replicate labels are counted across all files submitted in the "Metadata" category
having a "BioReplicate" or "Replicate" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses)
associated with this dataset.
The technical replicate count is defined as the maximum number of times any one distinct
combination of condition and biological replicate was analyzed across all files submitted in the
"Metadata" category. In the case of fractionated experiments, only the first fraction is
considered.
"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically
remapped by MassIVE to proteins in the
SwissProt
human reference database.
"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original
submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported
across all analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all
analyses (original submission and reanalyses) associated with this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses)
associated with this dataset.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison
across all analyses (original submission and reanalyses) associated with this dataset.
A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
It has been imported to MassIVE for reanalysis purposes, so its spectra data here may
consist solely of processed peak lists suitable for reanalysis with most software.