The aim of this study was to compare the signaling cascades initiated by the two closely related cytokines IL-2 and IL-15. Considering the relevance of protein tyrosine phosphorylation in signal transduction, in order to quantify changes in protein phosphorylation in response to IL-2 and IL-15, we combined triple SILAClabeling of leukemic T-cells with phosphotyrosine (pY)-specific antibody-based protein enrichment followed by mass spectrometry (MS) analysis. Following the strategy described above, we managed to decipher in detail the complex signaling networks triggered by IL-2 and IL-15. Interestingly, a large number of components of the three main signaling pathways known to be initiated in response to the interleukins (JAK/STAT; RAS/MAPK; PI3K/AKT) were identified.
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: SILAC ; signaling ; phosphotyrosine ; IL-2 ; IL-15
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Nerea Osinalde, Department of Biochemistry and Molecular Biology, Center of Experimental BioInformatic, University of Southern Denmark, N/A |
Submitting User: | ccms |
Osinalde N, Sanchez-Quiles V, Akimov V, Guerra B, Blagoev B, Kratchmarova I.
Simultaneous dissection and comparison of IL-2 and IL-15 signaling pathways by global quantitative phosphoproteomics.
Proteomics. 2015 Jan;15(2-3):520-31. Epub 2014 Sep 30.
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