MassIVE MSV000080767

Imported Reanalysis Dataset Public PXD001129

Dissection of IL-2 and IL-15 signaling pathways

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Description

The aim of this study was to compare the signaling cascades initiated by the two closely related cytokines IL-2 and IL-15. Considering the relevance of protein tyrosine phosphorylation in signal transduction, in order to quantify changes in protein phosphorylation in response to IL-2 and IL-15, we combined triple SILAClabeling of leukemic T-cells with phosphotyrosine (pY)-specific antibody-based protein enrichment followed by mass spectrometry (MS) analysis. Following the strategy described above, we managed to decipher in detail the complex signaling networks triggered by IL-2 and IL-15. Interestingly, a large number of components of the three main signaling pathways known to be initiated in response to the interleukins (JAK/STAT; RAS/MAPK; PI3K/AKT) were identified. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: SILAC ; signaling ; phosphotyrosine ; IL-2 ; IL-15

Contact

Principal Investigators:
(in alphabetical order) 		Nerea Osinalde, Department of Biochemistry and Molecular Biology, Center of Experimental BioInformatic, University of Southern Denmark, N/A
Submitting User: ccms

Publications

Osinalde N, Sanchez-Quiles V, Akimov V, Guerra B, Blagoev B, Kratchmarova I.
Simultaneous dissection and comparison of IL-2 and IL-15 signaling pathways by global quantitative phosphoproteomics.
Proteomics. 2015 Jan;15(2-3):520-31. Epub 2014 Sep 30.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.