MassIVE MSV000080085

Imported Reanalysis Dataset Public PXD003412

Contribution of primary human fibroblasts and endothelial cells to the hallmarks of inflammation as determined by proteome profiling - cytoplasmic proteins of untreated fibroblasts

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Description

While the most important players of inflammation have been well described, a systematic analysis of the proteins fulfilling the effector functionalities during inflammation has not yet been undertaken. Here we present a systematic proteome study of inflammatory activated primary human endothelial cells and fibroblasts. Cells were stimulated with interleukin 1-beta and fractionated in order to obtain secreted, cytoplasmic and nuclear protein fractions. Proteins were submitted to a data-dependent bottom up analytical platform using a QExactive orbitrap and the MaxQuant software for protein identification and label-free quantification. Results were further combined with similarly generated data previously obtained from the analysis of inflammatory activated peripheral blood mononuclear cells. Applying an FDR of less than 0.01 at both peptide and protein level, a total of 8235 protein groups assembled from 163858 peptides was identified. Comparative proteome analysis allowed us to determine proteins regulated in each kind of cells during inflammation. Remarkably, cells were working on similar inflammation-related tasks, however, by regulating different proteins. Thus, we were able to determine cell type-specific inflammatory signatures, apparently resulting from cell type-specific regulatory mechanisms. Hallmarks of inflammation emerged from these findings, representing commonly and cell type-specific responsibilities of cells during inflammation. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Cell type-specific inflammatory response ; hallmarks of inflammation ; mass spectrometry ; primary human fibroblasts and endothelial cells ; proteomics ; secretome

Contact

Principal Investigators:
(in alphabetical order) 		Dr Christopher Gerner
Submitting User: ccms

Publications

Slany A, Bileck A, Kreutz D, Mayer RL, Muqaku B, Gerner C.
Contribution of Human Fibroblasts and Endothelial Cells to the Hallmarks of Inflammation as Determined by Proteome Profiling.
Mol. Cell Proteomics. 2016 Jun;15(6):1982-97. Epub 2016 Mar 29.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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